血竭加味颗粒对自然衰老子宫肌瘤模型小鼠IGF-Ⅰ表达的影响

Effect of Xuejiejiawei Granule on Insulin-Like Growth Factor-I on Natural Aging Mouse Model of Uterine Hysteromyoma

目的:建立自然衰老和青年小鼠子宫肌瘤模型,研究血竭加味颗粒对子宫肌瘤模型小鼠子宫组织中胰岛素样因子Ⅰ(IGF-Ⅰ)的表达。方法:采用雌激素负荷法建立子宫肌瘤模型。将40只11月龄自然衰老小鼠、50只2～3月龄青年小鼠ICR雌性小鼠各随机分成5组,分别为模型组、对照组、血竭加味颗粒组、米非司酮组、桂枝茯苓胶囊组,通过观察子宫系数、子宫组织中IGF-Ⅰ的表达比较各组差异。结果:(1)血竭加味颗粒、米非司酮、桂枝茯苓胶囊均对雌激素负荷子宫肌瘤模型小鼠均有明显抑瘤作用。(2)血竭加味颗粒、米非司酮对自然衰老小鼠子宫组织IGF-Ⅰ的表达有显著抑制作用(P<0.01),桂枝茯苓胶囊对IGF-Ⅰ表达的抑制作用相对较小(P<0.05)。结论:血竭加味颗粒抑制子宫组织中IGF-Ⅰ的阳性表达可能为抑制子宫肌瘤的机制之一。

Objective：To study the effect of Xuejiejiawei granule on Insulin-lile Growth Factor-I（IGF-Ⅰ）on natural aging and young mouse models of uterine hysteromyoma.Methods：Uterine hysteromyoma models were established with estrogen load.Then forty eleven-month-old natural aging and fifty two to three-month-old young ICR female mouse were randomly divided into five groups,as a model group,control group,Xuejiejiawei granule group,mifepristone group ,Guizhi Fuling capsule group,uterine coefficients and expressions of IGF-I in uterine tissue were observed and compared among groups.Results：（1） Xuejiejiawei Granule,mifepristone and Guizhi Fuling capsule inhibited obviously the grouth of uterine hysteromyoma of mouse modles with estrogen load. （2） Xuejiejiawei Granule and mifepristone significantly inhibited expressions of IGF-I in uterine tissue of natural aging mouse（P〈0.01）,while the inhibitiones of GuizhiFuling capsule on IGF-Ⅰwere relatively small （P〈0.05）. Conclusion：Xuejiejiawei Granule inhibits expression of IGF-Ⅰin uterine tissue of mouse may one of the mechanisms inhibits the growth of uterine hysteromyoma.