野生狗牙根种质资源的AFLP遗传多样性分析

Genetic diversity of wild Cynodon dactylon germplasm detected by AFLP markers

利用AFLP标记对采自非洲以及中国四川、重庆、云南、贵州和西藏五省区的共44份野生狗牙根材料进行遗传多样性分析。10对引物共扩增出462个条带,其中多态性条带有452条,多态性条带比率为97.64%,材料间的遗传相似系数(GS)范围为0.64～0.95,平均GS值为0.76。根据研究结果进行聚类分析和主成分分析,可将供试材料分成五大类,分类结果与材料的地理分布大致相符,呈一定的地域性分布规律。由此可见,丰富的地理生态条件造就了狗牙根资源丰富的遗传多样性和明显的地域性分布规律。

Amplified fragment length polymorphism（AFLP）molecular markers were applied to detect the genetic variation of 44 agrestal accessions of Cynodon dactylon collected from Africa,Sichuan,Chongqing,Yunnan,Guizhou,and Tibet in China.A total of 462 bands were amplified by ten AFLP primer pairs from C.dactylon genetic DNA,of which 452 （97.64%） bands were found to be polymorphic.The AFLP-based genetic similarity values among 44 C.dactylon accessions ranged from 0.64 to 0.95,and the average Nei’s coefficient was 0.76.Analysis of cluster and principal component analysis showed that all the accessions could be distinguished by AFLP markers and divided into 5 groups.Accessions from the same area were almost all classified into the same group associated with their geographical distributions.Therefore,complex geographical ecological environments are important factors for genetic diversity and geographical distribution of C.dactylon.