棉纤维特异启动子LTP12驱动的基因phaB、phaC双价载体构建

Constructing expression vector of genes phaB and phaC drived by specific promoter LTP12 for cotton fiber

聚羟基丁酸酯(PHB)具有抗皱、保暖等特性,若棉纤维中存在PHB则棉纤维保暖性和抗皱性将会提高,因此通过基因工程技术手段将聚羟基丁酸酯的合成酶基因导入棉花中,就有可能在棉纤维发育过程中合成PHB,提高棉纤维的抗皱性、保暖性和防水等特性,从而达到改良棉纤维的品质。本研究分离了棉纤维特异启动子LTP12和聚羟基丁酸酯合成酶基因phaB和phaC,并构建了由LTP12驱动的基因phaB和phaC的双价植物表达载体pBLTPPBPC。

The poly-3-hydroxybutyrate（PHB） possesses anti-wrinkle and stay-warm characteristics.It has been hypothesized that the anti-wrinkle and stay-warm ability of cotton fiber would be improved if fiber contains PHB.The genetic engineered cotton plant containing the PHB synthesis would theoretically increase the incorporation of PHB into cotton fiber.As a result,the waterproof ability,anti-wrinkle and stay-warm characteristics of cotton fiber should be improved.In this study,the cotton fiber specific promoter LTP12 and two PHB synthesis genes （phaB and phaC） were integrated into a pBI121.Therefore,a new plant expression vector pBLTPPBPC was constructed.