期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

血红素氧合酶-2在新生大鼠延髓的表达及意义 被引量：1

Expression and significance of heme oxygenase-2 in the medulla oblongata of newborn rats
下载PDF
导出
摘要 目的探讨产生内源性一氧化碳(carbon monoxide,CO)的关键酶血红素氧合酶-2(heme oxygenase-2,HO-2)在新生大鼠延髓的表达及意义。方法采用新生Sprague-Dawley大鼠,行石蜡切片,分别进行HE染色和免疫组织化学染色(SP法),观察新生大鼠延髓神经核团的分布和不同神经核团中神经元的形态,以及新生大鼠延髓是否存在HO-2阳性神经元和阳性神经元的分布情况。结果在舌下神经核、疑核、巨细胞网状核、面神经核等部位均可见HO-2阳性神经元。结论 HO-2阳性神经元存在于新生大鼠延髓呼吸相关神经核团,提示内源性CO可能参与中枢性呼吸活动的调节。 Objective To test the expression and significance of heme oxygenase-2（HO-2）,a key enzyme of synthesizing carbon monoxide（CO）,in the medulla oblongata of newborn rats.Methods The experiments were carried out on the medullary slices of newborn Sprague-Dawley rats with paraffin section,the distribution of nuclei and the morphology of neurons in the medulla oblongata of newborn rats were observed with HE staining,the expression of HO-2 in the medulla oblongata of newborn rats was investigated with the immunohistochemical staining.Results HO-2-positive immunoreactive neurons could be observed in hypoglossal nucleus,ambiguous nucleus,gigantocellular reticular nucleus and facial nucleus in the medulla oblongata of newborn rats.Conclusion HO-2-positive immunoreactive neurons exist in the medullary respiratory related nuclei of newborn rats,which suggests that endogenous CO may be involved in the regulation of central respiratory activity.
作者 何颖 张洁 郑煜 丁艳 周华 唐玉红 陈丽
机构地区 四川大学华西基础医学与法医学院生理学教研室
出处 《西部医学》 2010年第6期985-986,990,共3页 Medical Journal of West China
基金 国家自然科学基金资助项目(NO.30770798)
关键词 血红素氧合酶-2 一氧化碳 新生大鼠 延髓 呼吸调节 Heme oxygenase-2 Carbon monoxide Newborn rat Medulla oblongata Respiratory regulation
分类号 R338.2 [医药卫生—人体生理学] Q24 [生物学—细胞生物学]
  • 相关文献

参考文献8

  • 1McCoubrey WK, Huang TJ, Maines MD. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heine oxygenase-3[J]. Eur J Biochern, 1997, 247: 725.
  • 2Wu LY, Wang R. Carbon monoxide: endogenous production, physiological functions, and pharmacological applications [J]. Pharmacol Rev, 2005, 57: 585-630.
  • 3Nakazawa K, Umezaki T, Zheng Y, et al. Behaviors of bullar respiratory interneurons during fictive swallowing and vomiting [J]. Head Neck Surg, 1999, 120: 412-418.
  • 4Kunibe I, Nonaka S, Katada A, et al. The neuronal circuit of augmenting effects on intrinsic laryngeal muscle activities induced by nasal air-jet stimulation in decerebrate cats [J]. Brain Res, 2003, 978: 83-90.
  • 5Li C, Guan ZL, Chan YS, et al. Projections from facial nucleus interneurons to the respiratory groups of brainstem in the rat [J]. Neurosci Lett, 2004, 368: 25-28.
  • 6严亨秀,张承武,郑煜.大鼠面神经核内非运动神经元的放电样式[J].华西医科大学学报,2002,33(2):189-191. 被引量：2
  • 7Zhang CW, Yan HX, Li C, et al. Possible involvement of the facial nucleus in regulation of respiration in rats [J]. Neurosei Lett, 2004, 367; 283-288.
  • 8赵凤臣,郭华,高晓群.大鼠延髓巨细胞网状核与脑干内脏运动核间的纤维投射研究[J].现代医学,2007,35(4):261-264. 被引量：2

二级参考文献10

  • 1李川,张承武,管振龙,陈应诚,郑煜.面神经核与呼吸调控初步探讨[J].四川生理科学杂志,2000,22(2):34-34. 被引量：2
  • 2Hermann G E, Holmes G M, Rogers R C, et al. Descending spinal projections from the rostral gigantocellular reticular nuclei complex[J]. J Comp Neurol,2003,455 (2) :210 -221.
  • 3Tanaka J, Arnold A P. An electrophysiological study of descending projections to the lumbar spinal cord in adult male rats [ J ]. Exp Brain Res,1993,96(1 ) :117-124.
  • 4Glucose U, Schrock H, Seller H, et al. Glucose utilization, blood flow and capillary density in the ventrolateral medulla of the rat [ J]. Pflugerts Arch, 1990,416 (5) :477-480.
  • 5Aicher S A, Reis D J, Ruggiero D A, et al. Anatomical characterization of a novel reticulospinal vasodepressor area in the rat medulla oblongata[ J]. Neuroscience, 1994,60(3 ) :761-779.
  • 6Jian B J, Acernese A W, Lorenzo J, et al. Afferent pathways to the region of the vestibular nuclei that participates in cardiovascular and respiratory control [ J ]. Brain Res, 2005,1044 ( 2 ) : 241-250.
  • 7Jakus J, Halasova E, Poliacek I, et al. Brainstem areas involved in the aspiration reflex: c-Fos study in anesthetized cats [ J ]. Physiol Res ,2004,53 (6) :703-717.
  • 8Mesulam M M. Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents [ J ]. J Histochem Cytochem, 1978,26 (2) : 106-117.
  • 9顾耀铭,陈以慈,叶鹿鸣.钨酸钠做为稳定剂的新的高灵敏HRP-TMB法——Ⅰ、光镜研究[J].神经解剖学杂志,1990,6(1):121-127. 被引量：39
  • 10李川,余微,郑煜.前包钦格复合体区微量注射氨基酸类药物的呼吸效应[J].生理学报,2000,52(4):333-337. 被引量：5

共引文献2

同被引文献11

  • 1Kikuchi G, Yoshida T, Noguchi M. Heme oxygenase and heme degradation[J]. Biochemical and Biophysical Research Communications, 2005, 338 (1): 558-670.
  • 2Seta F, Bellner L, Rezzani R, et al. Heine oxygenase-2 is a critical determinant for execution of an acute inflammatory and reparative response[J]. American Journal of Pathology, 2006, 169(5):1 612-1 623.
  • 3Cao J, Puri N, Sodhi K, et al. Apo A1 Mimetic Rescues the Diabetic Phenotype of HO-2 Knockout Mice via an Increase in HO-1 Adiponectin and LKBI Signaling Pathway[J]. Interna- tional Journal of Hypertension, 2012, 2012: 628147.
  • 4Pratt T, Roger T , Laura E, et al. BrianExposure Kupffer Ceils and in Mice after Chronic Ethanol TLR4/MyD88-Inde- pendent Signaling in Rat Adiponectin and Heme Oxygenase-1 Suppress[J]. The Journal of Immunology, 2002, 22 (12) : 1-8.
  • 5Alfonso Loeches S, Pascual Lucas M, Blanco A M, et al. Piv- otal role of TLR4 receptors in alcohol-induced neuroinflamma- tion and brain damage[J] The Journal of Neuroscience,2010,30(24) :8 285-8 295.
  • 6Gong P, I-lu B,Stewart D, et al. Cobalt induces heme oxygen- ase-1 in human pulmonary epithelial cells results in cell growth arrest and increased to hyperoxia[J]. Proceedings of the Na- tional Academy of Sciences of the United States of America, 1996,93:10 393-10 398.
  • 7Basuroy S, Bhattacharya S, Tcheranova D. HO-2 provides en- dogenous protection against oxidative stress and apoptosis caused by TNF-a in cerebral vascular endothelial cells[J]. A- merican Journal of Physiology-Cell Physiology, 2006,291 : 897- 908.
  • 8Dore S, Takahashi M, Snyder S H, et al. Bilirubin, formed by activation of heme oxygenase-2, protects neurons against oxidative stress injury[J]. Proceedings of the National Acade- my of Sciences, 1999, 96:2 445-2 450.
  • 9Takeuchi O and Akira S. Pattern recognition receptors and inflammation[J]. Cell, 2010, 140(6): 805-820.
  • 10Seta F, Bellner L, Rezzani R, et al. Herne oxygenase-2 is a critical determinant for execution of an acute inflammatory and reparative response[J]. American Journal of Pathology, 2006, 169(5):1 612-1 623.

引证文献1

西部医学
2010年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部