胶体金免疫层析快速检测技术及其在水产养殖业中的应用前景

Progress and prospect of colloidal gold-based immunochromatographic lateral flow assay and its application in aquaculture

建立快速、准确、简便易行的病害早期快速检测技术,是从事动物疫病防控研究工作者和产业界人士的迫切愿望。自从开辟免疫分析技术以来,越来越多更灵敏、更便捷的免疫分析方法被开发出来,以胶体金免疫层析法为基础建立起来的快速检测试纸,被认为是当前最快捷、高敏感的一种免疫学检测技术。该项技术真正实现了快速简易检测靶目标,并已在畜牧业中得到普及应用,成为当前畜禽病害早期防控的最有效方法之一。本文就该项技术在畜牧兽医领域的研究进展、应用现状、发展前景进行了介绍、分析和讨论,旨在为水产养殖动物病害的早期诊断提供借鉴,以期获得一种适用于水产动物病害的、专用的快速检测新技术和新产品。

Immunoassays are demonstrated to be simple,rapid,and cost effective.For the past decades,more and more techniques are being developed which combine the advantages of approaches in highly powerful analytical tools.The convenience and speed of the test have been achieved by a novel concept of colloidal gold-based immunochromatographic assay,which depends on the transport of a color-labeled reactant to its binding partner immobilized on the surfaces of the membrane.This new technique has been proved to be a well-established and appropriate technique for a variety of point-of-care and field-use applications,which was widely used as a convenient test for human being and terrestrial animal diseases in nowadays.In this paper,progress on the study of this new technique has been summarized in detail,which focuses on its principle,application and development.Based on these,its practicability in aquatic animal is also introduced.It would provide a new point of view on the innovation of simple and fast detection of disease in aquaculture.