恙螨体内出血热病毒检测增殖及其定位

Detection and Proliferation of Hemorrhagic Fever Virus in Chigger Mites

目的用分子生物学方法研究小盾纤恙螨的媒介意义。方法聚合酶链反应和原位分子杂交法。结果从恙螨体内检测到肾综合征出血热病毒ＲＮＡ；取恙螨幼虫、若虫定期做肾综合征出血热病毒ＴＣＩＤ５０／ｍｌ滴定，证明恙螨体内肾综合征出血热病毒可传播并有增殖现象；用原位分子杂交技术在恙螨的卵巢细胞、支肠细胞等组织中检测到肾综合征出血热病毒ＲＮＡ。结论研究结果为恙螨的媒介意义提供了具有分子水平的直接证据，对肾综合征出血热的流行病学和预防具有重要的理论意义和实用价值。

Objective To study the impact of Leptotromhidium scutellaris as a vector in transmission of hemorrhagic fever. Methods Reverse transcription polymerase chain reaction (PCR) and in situ hybridization technique were used to detect RNA of hemorrhagic fever with renal syndrome virus (HFRSV). TCID 50 /ml of HFRSV were titrated for larva and nymph of chigger mites periodically. Results RNA of HFRSV was detected in the tissues of chigger mites, such as ovary cells, etc. by PCR and in situ hybridization. Titration of TCID 50 /ml showed that HFRSV could be transmitted transstadially and porliferated in chigger mites. Conclusion It provides direct evidence at molecular level for the role of chigger mite as a vector in transmission of HFRSV with theoretical and practical importance in prevention of HFRS.