Graves病T、B细胞功能异常及甲巯咪唑体外调节作用的研究

T and B Cells Abnormalities in Graves'Disease Patients and the Modulating Effect of Methimazole

目的：探讨Ｇｒａｖｅｓ病（ＧＤ）患者Ｔ、Ｂ细胞异常及甲巯咪唑体外对它们的影响。方法：将２５例ＧＤ病人 外周血单个核细胞（ＰＢＭＣ）在不同浓度的甲巯咪唑条件下进行体外细胞培养６天后，同时检测Ｔ细胞亚群及ｓＩＬ－ ２Ｒ和ＩｇＧ的含量。结果：ＧＤ病人ＰＢＭＣ中的ＣＤ_８~＋Ｔ细胞亚群百分率明显低于对照组，ＣＤ~＋_４／ＣＤ_８~＋比值显著升高 （ｐ＜０．０１）。 ｓＩＬ－２Ｒ及ＩｇＧ水平较对照组显著升高（ｐ＜０．０１）；Ｘ１０^（－５）ｍｏｌ／Ｌ的甲巯咪唑可明显升高ＧＤ患者ＰＢ－ ＭＣ培养后ＣＤ_８~＋ Ｔ细胞的百分率（Ｐ＜０．０１），降低ＣＤ_４~８ＣＤ_８~＋比值及ｓＩＬ－２Ｒ和ＩｇＧ水平（Ｐ＜０．０１）。提示：ＧＤ病 人Ｔ细胞亚群对免疫应答发生调节失衡，甲巯咪唑具有调节ＧＤ患者Ｔ、Ｂ细胞功能异常的作用。

In order to clarify whether there were T and B cells abnormalities in Graves' disease (GD ) patients and the effect of methimazole(MMI ) on them, peripheral blood mononuclear cells (PBNC) isolated from GD patients peripheral blood were incubated for 6 days at absent or present of different concentrations of MMI. Normal individuals'PBMC were also cultured as control. The culture supernatant were used for detecting. IgG and soluble interleukin-2 receptor(sIL-2R)and cell pellet were assayed for detecting PBMC surface CD molecules. The results showed that: compared to normal individual group, in PWM induced PBMC of GD patients, the percentage of CD8+ T subset was decreased and CD4+ /CD8+ cell ratio was increased. In PBMC culture supernatant it showed high level of sIL-2R and IgG, all there parameters had significant statistted value(p<0.01). In GD patients PBMC which were cultured with ×10-5 mol/L MMI,CD8+ T cell percentage was significantly higher, the CD4+ /CD8+ cell ratio, sIL-2R and IgG level were declined (P< 0.01 ). There results demonstrated that in GD, the regulation of immune response by T cells subpopulation was in unbalanCe characterizing in predominently high reactivity. Furthermore it is suggested that in GD, there were not only B cell but also T cell function abnormalities, and MMI treatment may turn the abnormal function to normal.