血管内皮细胞生长因子表位肽体外抗肿瘤作用研究

Anti-tumor effect of epitopic peptides of vascular endothelial growth factor in vitro

目的:研究筛选血管内皮细胞生长因子(VEGF)不同表位肽抗肿瘤及抗血管新生效用。方法:构建4种不同的pCANTAB5-E/VEGF表位肽载体,将VEGF表位肽通过噬菌体展示技术展示于噬菌体表面,MTT法检测VEGF噬菌体表位肽对黑色素瘤细胞(B16)、人脐静脉血管内皮细胞(HUVEC)增殖的影响,Transwell小室检测其对B16F10细胞迁移的影响,ECMgel检测其对HUVEC成管的影响。结果:筛选出VEGF的4种(VSB、VNB、VEB、VTB)表位肽中,VNB对HUVEC细胞增殖的抑制率达到64.8%,VEB、VTB对B16的增殖抑制率分别为75.7%和66.5%。4株表位肽对高转移细胞B16F10迁移抑制率均超过80%。结论:筛选得到的4株VEGF表位肽对黑色素瘤细胞、血管内皮细胞的增殖、迁移和成管有抑制作用,通过分析其表位区段后可制备成小分子多肽,为今后抗肿瘤多肽药物和疫苗的研发奠定基础。

Objective：This study was to get several peptides of vascular endothelial growth factor（VEGF） which were designed by computer simulation and the specific anti-tumor peptides were selected to use in tumor inhibition.Methods：The peptides were designed and displayed in the surface of phages by using phage display system.Anti-tumor and anti-vascular endothelial cell effects of the peptides on B16 and human umbilical vein endothelial cells（HUVEC） were analyzed with MTT assay.Tumor cell migration assay was applied to analyze the activities of the peptides with Transwell model on B16F10 cells.Vascular endothelial cell tube formation assay was applied to analyze the effects of the peptide on HUVEC with ECM gel model.Results：It was shown by in vitro experiments that 4 peptides had anti-tumor functions against B16,especially VEB and VTB,whose inhibitory rate were 75.7% and 66.5%.Besides,the inhibitory rate of VNB to HUVEC was 64.8%.All of the peptides had significant effect of anti-migration in B16F10 and the anti-migration rate was beyond 80%.Conclusion：The peptides have significant inhibitory effects on proliferation,migration and tube formation of Melanoma cells and vascular endothelial cells in vitro.They are probably helpful to develop new anti-tumor drugs and anti-tumor vaccines in the future.