中国经采供血HIV感染长期不进展者CD4^+T淋巴细胞趋化因子受体表达研究

Correlation between chemokine receptor expression on CD4^+T cells and disease progression in HIV-infected long term nonprogressors of China

目的:了解中国经采供血HIV感染长期不进展者CD4+T淋巴细胞趋化因子受体表达,分析其与疾病不进展的关系。方法:收集43例经采供血HIV感染长期不进展者、82例无症状HIV感染者、35例AIDS病人及40例健康对照的抗凝全血,用流式细胞仪检测趋化因子受体CCR5、CXCR4的表达,并分析其与病毒载量、CD4+T淋巴细胞绝对值及T淋巴细胞活化的相关性。结果:长期不进展组CD4+T细胞表面CCR5的表达明显低于无症状HIV感染组及AIDS组(P<0.01),与健康对照无显著差异;CD4+T细胞表面CXCR4的表达各组无显著差异。CD4+T细胞表面CCR5的表达与CD4+T细胞数量显著负相关(r=-0.498,P<0.05),与病毒载量无显著相关性。CD4+T细胞表面CCR5的表达与HLA、CD38在CD4+、CD8+T细胞的表达水平显著正相关(P<0.001,CD38在CD4+T细胞的表达除外),CD4+T细胞表面CXCR4的表达与HLA在CD4、CD8+T细胞的表达水平显著负相关(P<0.01)。结论:HIV感染长期不进展者CD4+T细胞趋化因子受体CCR5表达维持较低水平,与疾病不进展相关。

Objective：To investigate the expression of coreceptors CCR5 and CXCR4 on CD4 T lymphocytes of HIV-infected long term non porgressors（LTNP） of former blood donors in China.Methods：43 HIV infected LTNPs,82 asymptomatic HIV infected patients,35 AIDS patients and 40 normal controls were enrolled and three color flow-cytometry was used to detect the expression of coreceptors CCR5 and CXCR4 in whole blood samples taken from the patients and the controls.Results：The level of CCR5 expression on CD4^＋T lymphocytes of LTNPs was lower than that of asymptomatic HIV-1 infected patients and AIDS patients（P〈0.01）.The CCR5 expression on CD4^＋ T lymphocytes of HIV-1 infected patients was significantly correlated with the number of CD4^＋ T cells（r=-0.498,P〈0.05）,and with the activation of CD4^＋,CD8^＋ T cells（P〈0.01）.Conclusion：Decreased expression of CCR5 on CD4^＋T cells of HIV infected long term non progressors might contributes to the establishment of the stable immunological course of the disease.