影响降解菌Y_1降解土壤中异噁草酮的三种因素的优化

Optimization of Three Factors Affecting Clomazone Degradation in Contaminated Soil by Degrading Bacteria Y_1

【目的】研究田间持水量、接种量以及肥料添加量对降解菌Y1降解土壤中异噁草酮的影响,优化异噁草酮降解菌Y1的降解条件。【方法】利用生物测定的方法,通过二次回归旋转组合设计确定最优降解条件。【结果】得到降解条件的优化数学回归模型为y=65.05812+9.60065*C1+10.49792*C2+3.60229*C3-6.88851*C12-4.41187*C22。各因素对降解率的影响大小顺序依次为接种量、田间持水量、肥料添加量。【结论】当接种量(13.46—15.48 mL.kg-1),田间持水量(56.93%—62.0%),肥料添加量(1.82—2.18 g.kg-1)分别在所示范围内时,降解菌在异噁草酮有效成分浓度为500μg.kg-1的风干土壤中,30 d后的降解率可达到60%以上。

【Objective】 For optimization of clomazone degradation in contaminated soil by degrading bacteria Y1,three factors affecting degradation rate including field moisture capacity,the amount of inoculum and fertilizer used were studied.【Method】 Based on the bioassay method,the degradation rates of bacteria Y1 under different conditions were compared by using orthogonally rotational combination design.【Result】 A mathematical regression model for clomazone degradation was established,that is,y= 65.05812＋9.60065＊C1＋10.49792＊C2＋3.60229＊C3-6.88851＊C12-4.41187＊C22.Results indicated that the order of significance affecting degradation rate is： the amount of inoculumthe field moisture capacitythe amount of fertilize used【.Conclusion】When the amount of inoculum was 13.46—15.48 mL.kg-1,the field moisture capacity was 56.93%—62.0% and the amount of fertilize used was 1.82—2.18 g.kg-1,the degradation rate was over 60% in air-dried soil contained clomazone 500 μg.kg-1after 30 days.