反相高效液相色谱法测定化妆品中鞣花酸

Determination of ellagic acid content in cosmetics by reversed-phase high performance liquid chromatography

采用反相高效液相色谱法测定化妆品中鞣花酸的含量。色谱条件为：Venusil XBP-C18（4.6 mm i.d×250 mm,5μm）色谱柱,流动相V（乙腈）∶V（水）∶V（三氟乙酸）=20∶80∶0.08,流速1.0 mL/min,检测波长254 nm。结果表明,在此条件下鞣花酸在3.98μg.mL-1-79.6μg.mL-1范围内与相应的峰面积具有良好的线性关系（R2=0.999 8）,线性回归方程为A=203.83ρ-2.462 7,回收率在96.5%-101.5%,RSD为0.6%。

A reversed-phase high performance liquid chromatographic method for the determination of ellagic acid in cosmetics was established.The sample was analyzed on a Venusil XBP-C18 column（4.6 mm i.d×250 mm,5 μm） with UV 254 nm.The mobile phase consisted of V（CH3CN）∶V（H2O）∶V（CF3COOH）=20∶80∶0.08,at a flow rate of 1.0 mL/min.Results showed that as the concentration of ellagic acid is in the range of 3.98 μg·mL-1~79.6 μg·mL-1,the relationship between concentration and the corresponding peak area shows good linearity（R2=0.999 8）.The regression equation can be expressed as A=203.83ρ-2.462 7;and the recoveries of ellagic acid achieve 96.5%~101.5%.The RSD of the method is 0.6%.