枸杞多糖对淋巴细胞功能的影响

Effect of lycium bararum polysaccharides on blood lymphocytes function

目的研究枸杞多糖（LBP）对正常人外周血淋巴细胞免疫调节功能的影响。方法无菌分离人外周血淋巴细胞，加入植物凝集素（PHA）和不同质量浓度的LBP。MTT法检测其对淋巴细胞增殖的影响；ELISA法检测LBP作用后淋巴细胞培养上清液中肿瘤坏死因子-α（TNF—α）的表达情况。结果50～100mg／LLBP能明显增强PHA活化的人外周血淋巴细胞的增殖；LBP能促进淋巴细胞产生TNF—α，并呈现浓度依赖性。结论LBP能增强人外周血淋巴细胞的免疫功能，发挥抗肿瘤作用。

Objective It is to explore the effect of lycium bararum polysaccharides（LBP） on immunoregulation of human peripheral blood lymphocytes. Methods Lymphocytes from human peripheral blood were isolated and coincubated with phyto- hemagglutin （PHA） and different concentration of LBP. Proliferation of lymphocytes was detected with MTT. TNF-α secretion in supernatant of lymphocytes culture after LBP treatment were determined with ELISA assay. Results The proliferation of lymphocytes was enhanced by 50 - 100 mg/L LBP after activation of the proliferation of lymphocytes from human peripheral blood with PHA in a dose dependent manner （P 〈 0.01 ）. The production of TNF -α by lymphocytes could be augmented by LBP treatment in a dose dependent manner （ P 〈 0.01 ）. Conclusion LBP could enhance the immune response of lymphoeytes from human peripheral blood, which may be related with killing carcinoma cells.