微波烧结墨鱼骨作为骨组织工程支架材料的体外研究

Experimental study of Microwave sintering cuttlebone as bone tissue engineering scaffolds in vitro

目的:探讨微波烧结墨鱼骨作为骨组织工程支架材料的可行性。方法:墨鱼骨微波烧结后在扫描电子显微镜下观察,根据图像计算总孔隙率及开孔率;进行XRD表征,细胞毒性实验,人骨髓间充质干细胞黏附、活力及生长状态的观察,浸提液的成骨诱导培养实验。结果:微波烧结后墨鱼骨的有机质可被完全碳化,骨盐成分及适合发挥成骨效能的三维微孔结构得以保存,体外细胞培养无毒性,人骨髓间充质干细胞可在其表面细胞黏附生长,其浸提液可显著促进干细胞的成骨分化。结论:微波烧结的墨鱼骨具有作为骨组织工程支架的一些重要特性如无细胞毒性、良好的生物相容性、良好的表面活性及适合发挥成骨效能的多孔骨盐结构等;利用微波技术可完全碳化墨鱼骨有机质,灭活抗原性及灭菌可同时进行。因此,微波烧结的墨鱼骨具有作为骨组织工程支架的潜在可能性。

Objective：To explore the feasibility of microwave sintering cuttlebone as bone tissue engineering scaffolds in vitro. Meth- ods：The microwave sintering cuttlebone were evaluated by scanning electron microscope and XRD, and the total porosity and open -cell rate were calculated. We also evaluated its cytotoxicity and the proliferation and differentiation of human bone marrow mesenchymal slem cell （hMSCs） on it. Results：Microwave sintering cuttlebone can be completely carbonized to remove organic matter, the porous structure suitable for the osseointegration and bone salt elements are maintained and non - cytotoxic. The human bone marrow derived mesenchymal stem cells （hMSCs） could growth on its surface. The extracts had benefit for ostegeneic differentiation of hMSCs. Conclusion：The micro- wave sintering cuttlebone could be the scaffold of bone tissue engineering with a number of important features such as no Cylotoxicity , good biocompatibility, well surfactant and porous structure suitable for the osseointegration. The organic matter of cuttlebone can be completely carbonized by the technology of microwave sintering which effectively inactivate the antigen and microbial origin. Therefore, microwave sin- tering cuttlebone have potential as a scaffold for bone tissue engineering.