通用引物检测宫颈脱落细胞中病毒的临床应用

Clinical application of polymerase chain reaction-endonuclease cleavage in detecting human herpesvirus of cervix

目的：探讨通用引物检测多种病毒的PCR方法。方法：应用聚合酶链反应-酶谱法（PCR－EC）和疱疹病毒通用引物，检测宫颈脱落细胞单纯疱疾病毒Ⅰ型（HSV—Ⅰ）、单纯疱疹病毒Ⅱ型（HSV—Ⅱ）、爱泼期坦—巴尔病毒（EBV）和人5细胞病毒（HCMV）。结果：健康人（20例）和宫颈糜烂患者（34例）。HCMV阳性检出率分别为10．0％和41．2％，两者差异显著（P＜0．05）；HSV—Ⅰ分别为5．O％和及14．7％；HSV—Ⅱ分别为1l％和20．6％；FBV分别为15．0％和17．6％；可检测到1fg的DNA。结论：通用引物PCR－EC检测HCMV、HSVⅠ、HSVⅡ、EBV，具有敏感、特异、便捷、快速、经济的特点，对病毒感染早期快速诊断及流行病学研究提供了科学的方法。

Objective: To study a PCR mothed which can detect more than one kind of virus infection.Methods: Polymerase chain reaction-endonuclease cleavage and single pair of ollgomucleotide primers selectedwithin a highly conserved region of DNA polymerase gene of the herpesvirus were used in detecting human cytomegalovirus (HCMV),herpes simplex virus Ⅰ,Ⅱ (HSV Ⅰ,HSV Ⅱ) and Eptein-Barr virus (EBV). Results: Twenty healthy women and 34 cervicitis were included in the study. The POsitive rates of HCMV inhealthy and cervicitis were 10. 0% and 41. 2% respectively (P<0' 05). The POSitive rates of HSV I,HSV E,EBV was 5. 0%, 10. 0%, 15. 0% in healthy and 14. 7 %, 20. 6%, 17. 6% in cervicitis,respectively. ~fusion:Detcting HCMV,HSV I,HSV E and EBV by single Pair of oligonucleotide primers selected within a highlyconserved region DNA polymetase gene Of the herpesvirus PCR-EC is sensitive,spesific,rapid and ecorlodric. Itprovided a scientific method in early diagnosis Of virus infection and epidentiology study.