小麦全蚀病拮抗木霉ZBS6的分离、筛选及鉴定

Isolation,Screening and Identification of Antagonistic Trichoderma ZBS6 to Wheat Take-all

从河南省农科院试验地小麦田土中分离获得12株木霉,通过室内对峙试验,筛选出1株对小麦全蚀病菌拮抗作用较强的木霉菌株ZBS6,室内测定其抑菌率和寄生率分别达到82.5%和80%。并对该拮抗木霉进行了抑菌谱测定,研究了不同培养条件对该木霉菌株菌丝生长及产孢量的影响,同时利用形态学及分子鉴定相结合方法对木霉菌株ZBS6进行了鉴定。结果表明:ZBS6具有广谱的抑菌活性。其最适培养条件:以葡萄糖为碳源,基质酸碱度(pH)5.0,温度为25℃,光暗交替培养;光照处理对菌丝生长影响不明显,但显著促进产孢量。结合形态学特征及rDNA ITS序列聚类分析结果,ZBS6鉴定为绿色木霉(Trichoderma viride)。

Twelve Trichoderma isolates were isolated from wheat fields in Henan Academy of Agricultural Sciences.Through indoor opposing test we isolated one Trichoderma strain ZBS6,which showed strong antagonism.Its antifungal and parasite rate can reach 82.5% and 80%,respectively.Furthermore,we assayed its antifungal range as well as hypha growth and sporification impact under different culture condition.Meanwhile we identified the Trichoderma strain ZBS6 using morphological and molecular methods.The results are as follows：ZBS6 has wide range antifungal activity and the optimum culture condition is glucose as carbon source,pH 5.0,25℃,light and dark alteration.No distinct effect was seen by light treatment,but sporification was increased apparently.Combined with morphological characteristic and cDNA ITS sequence alignment analysis,ZBS6 is identified as Trichoderma viride.