大叶茜草素在甲醇溶液中的稳定性研究

Study on the stability of mollugin in methanol solution

目的考察在光照、加热条件下甲醇溶液中大叶茜草素的稳定性。方法以甲醇-水-四氢呋喃(310:90:3)为流动相,利用DiscoveryC18色谱柱在250nm处测定大叶茜草素的含量,考察在4500±500lx光照、室内自然光、不同温度(30、40、50℃)条件下甲醇溶液中大叶茜草素的稳定性。结果光照条件下大叶茜草素的含量降低明显,避光、加热条件下的含量不变。结论甲醇溶液中的大叶茜草素在光照下不稳定,测定其含量应避光操作。

OBJECTIVE To study the stability of mollugin in methanol solution under light and heated conditions.METHODS The determination was carry out on a Discovery C18 column at 35 ℃ with methanol-water-THF（310：90：3） as mobile phase and detection wavelength at 250 nm.The stability of mollugin was studied by HPLC under light irradiation（4500 ± 500 lx）,indoor nature light and heated at 30 ℃,40 ℃,50 ℃ temperature.RESULTS Under light,the content of mollugin in the methanol solution decreased evidently.When kept away from light or heat,the content of mollugin didn＇t show evident change.CONCLUSION The mollugin in methanol solution was found to be unstable under light condition and the determination should be kept away from light.