渗透调节提高种子活力的机理研究

STUDIES ON MECHANISM OF INCREASING SEEDS VIGOR BY OSMOCONDITIONING

种子在吸胀萌发期间存在多条呼吸途径和电子传递途径,大豆种子在25%PEG溶液(ψ_w=-1.1860 MPa)中缓慢吸胀与在清水中快速吸胀其呼吸代谢明显不同。缓慢吸水可使EMP-TCA途径的强度提高,并保持较高的细胞色素电子传递途径活性,快速吸水种子EMP途径的上升可能与发酵作用有关;酶活性测定表明,缓慢吸水种子中的总脱氢酶、琥珀酸脱氢酶、细胞色素氧化酶、过氧化氢酶活性提高。

There were multiple respiratory pathways and electron transport pathways duringimbibition and germination of seeds. The respiratory metabolism of soybean seeds in 25%PEG solution (ψ_w=-1.186 MPa) was quite different from that in pure water. During slowimbibition, the activity of EMP-TCA respiratory pathway of seeds was increased and highactivity of cytochrome electron transport pathway was maintained. In rapidly imbibedseeds the rise of EMP pathway could be related to fermentation. The experiment alsoshowed that the activity of total dehydrogenase, succinic dehydrogenase, cytochromeoxidase and catalase of seeds was increased with PEG treatment.