天麻抗真菌蛋白基因转化辣椒的研究

Study on Transformation of Gastrodia Antifungal Protein Gene in Pepper

为获取辣椒的抗真菌种质资源,运用RT-PCR技术扩增出GAFP的cDNA序列,通过基因工程技术构建植物表达载体pBI121-GAFP,并导入遵椒1号辣椒。转化后再生的植株经PCR检测,初步证实GAFP基因已经整合到辣椒基因组中,得到了GAFP基因转化遵椒1号辣椒植株。

The cDNA sequence of Gastrodia antifungal protein（GAFP） amplified by RT-PCR was directly transformed into Zunjiao 1（pepper variety） by plant expression vector pBI121-GAFP under genetic engineering technique.The GAFP gene was successfully transformed into pepper genome of Zunjiao 1 after PCR determination of transformed regeneration plantlets of Zunjiao 1.