摘要
为了研究低温预处理不同时间对烟草花药培养胚状体诱导率的影响,以烟草K326×GDH黄叶突变体组合的F2、F3和F4植株的花药为试验材料,在接种前将花药用4℃低温预冷处理24h、48h和72h后,在(26±1)℃条件下进行花药培养。结果表明,烟草花药经4℃低温预处理48h后接种,可明显提高花药胚状体的诱导率,最佳培养世代为F2。
The anthers of F2,F3 and F4 plants of K326×GDH（the mutant with yellow leaf） pretreated at 4℃ for 24h,48h and 72h were cultured at（26±1）℃ to study the effect of different low temperature time on embryoid induction rate of tobacco anthers.The results showed that culture of tobacco anthers pretreated at 4℃ for 48h could significantly increase the embryoid induction rate and the optimum culture generation was F2.
出处
《贵州农业科学》
CAS
北大核心
2010年第5期16-18,共3页
Guizhou Agricultural Sciences
基金
贵州省"十一五"重大专项"贵州烤烟种质资源创新及优质抗病新品种选育"[黔科合重大专项字(2006)6026]
贵州省烟草专卖局(公司)"十一五"重大专项"烤烟新品种选育及配套技术研究"[黔烟科(2006-03)]
关键词
烟草
出苗率
低温处理
花药培养
tobacco
emergence rate
low temperature treatment
anther culture
