HPLC法测定消糜栓中苦参碱的含量

Determination of matrine in Xiaomishuan by HPLC

目的建立HPLC法测定消糜栓中苦参碱的含量。方法采用天津兰博C18色谱柱,流动相：甲醇-水-三乙胺（45：55：0.275）,流速为1.0 ml·min^-1,检测波长：220 nm。结果线性范围80-400 mg·L^-1（r=0.9995）。平均回收率为98.7%（n=6）,RSD为1.22%。结论本方法简便、快速、重复性好,可用于该制剂的质量控制。

Aim To establish an HPLC method for determination of matrine in Xiaomishuan.Methods The LabAlliance C18 column with methanol-water-triethylamine（45：55：0.275）was used as the mobile phase.The detection flow was 1.0 ml·min^-1.The detection wavelength was at 220 nm.Results The standard curves were in good linear relationship at the rang e from 80 to 400 mg·L^-1,r=0.9995.The average recovery was 98.7%（n=6）with RSD of 1.22%.Conclusions The method is simple and accurate with good reproducibility and can be used for the quality control.