烤烟烟叶浸出物对微生物生长的影响

Effect of Aqueous Extract from Flue-cured Tobacco Leaf on Growth of Microbes

采用比浊法和菌丝体湿重法测定生物量,测定了初烤烟叶蒸馏水浸出物对4种细菌和3种真菌生长的影响.结果表明,烟叶浸出物对细菌和真菌生长的影响不同.在剂量范围内,烟叶浸出物对铜绿假单孢菌(Pseudo-monas aeruginosa),大肠杆菌(Escherichia coli),枯草芽孢杆菌(Bacillus subtilis)和金黄色葡萄球菌(Staphylococcusaureus)没有抑制作用,对爪哇毛霉(Mucor jaranicas),灰葡萄孢(Botrytis cinerea)和白色念珠菌(Canadida albi-cans)有抑制作用,抑制率分别为89.5%,74.8%和19.4%.

Effect of aqueous extract from flue-cured tobacco leaf on growth of Pseudomonas aeruginosa,Escherichia coli,Bacillus subtilis,Staphylococcus aureus,Canadida albicans,Botrytis cinerea and Mucor jaranicas were investigated in vitro experiments.Differences were found between the effects of bacteria and fungi in our designed dosage.Aqueous extract from flue-cured tobacco leaf inhibited 89.5% growth rate of Mucor jaranicas,74.8% of Botrytis cinerea and 19.4% of Canadida albicans respectively,while the aqueous extract had no inhibitory effects when applied to Pseudomonas aeruginosa,Escherichia coli,Bacillus subtilis and Staphylococcus aureus respectively.The result can help us find a new way to reuse the waste tobacco leaves.