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链脲佐菌素诱导培养皮层神经细胞损伤作用的观察

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摘要 目的研究链脲佐菌素(STZ)对原代培养大鼠皮层神经元是否具有损伤作用。方法常规原代神经细胞培养的方法,培养新生1 d^3 d的Wistar大鼠皮层神经元,通过检测CCK-8,乳酸脱氢酶(LDH)释放检测和calcein-AM染色,观察加入不同浓度STZ(1μmol/L、10μmol/L、100μmol/L、1 000μmol/L)36 h后对细胞损伤的作用。结果 STZ处理后对原代培养大鼠皮层神经细胞产生明显的损伤作用,包括细胞活力的下降,活细胞数目的减少,LDH释放的增加并呈现剂量依赖性。结论 STZ对离体培养的神经细胞具有类似脑室注射同样的神经损伤作用。
作者 马国英 杨小荣 赵欣 秦华平 史瑞红 王晔 张策
机构地区 山西大同大学医学院 山西医科大学
出处 《中西医结合心脑血管病杂志》 2010年第6期719-720,共2页 Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金 山西省基础研究项目(项目名称:Humanin拮抗NMDA诱导的神经毒作用机制-MAPKs通路分析)
关键词 培养神经细胞 链脲佐菌素 损伤
分类号 R378 [医药卫生—病原生物学]
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参考文献12

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中西医结合心脑血管病杂志
2010年 第6期

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