摘要
FAV-Ⅷ型禽腺病毒口服接种感染2日龄SPF雏鸡,攻毒后取不同日龄的肝脏于福尔马林溶液中固定,并进行石蜡包埋,石蜡切片进行原位杂交染色。结果表明,着染部位主要出现在坏死灶或血管周围的成团或散在的淋巴细胞的胞浆内,在病情严重时的5dpi开始增多,至20dpi表达量也很高,以后开始减少,直到30dpi基本消失。说明鸡包涵体肝炎过程中肝脏IFN-γ的mRNA大量上调,可能与T淋巴细胞的增多有关;而且IFN-γ作为免疫增强剂与抗病毒感染和病症恢复也有关。
Two-day-old SPF chickens were orally challenged with fowl adenovirus-VIII （FAV-VIII）.Then their livers were collected at different days post inoculation （dpi） and fixed in formalin.After paraffin embedding,paraffin section was stained by in situ hybridization.The results showed that staining mainly appeared in cytoplasm of sporadic or conglobate lymphocytes around necrotic foci or blood vessels.IFN-gamma mRNA became more at 5 dpi and was high at 20 dpi.It then decreased and disappeared at 30 dpi.This indicated that the mass ascent of IFN-gamma mRNA during the course of avian inclusion body hepatitis may be related to the increase of T lymphocytes.This also indicated that IFN-gamma,an immunopotentiator,was related to antivirus and recovery.
出处
《畜牧与饲料科学》
2010年第3期1-2,7,共3页
Animal Husbandry and Feed Science
关键词
包涵体肝炎
原位杂交
T淋巴细胞
IFN-Γ
inclusion body hepatitis
in situ hybridization
T lymphocytes
IFN-gamma
