淋巴造影剂专利蓝脂质体的制备及其生物学表征

Patent-blue-liposomes for lymphography and the detection of their biological property

目的 制备淋巴造影剂专利蓝脂质体（PB-LPS）.方法 采用被动载药法制备PB-LPS.选用Wistar大鼠、在后足掌皮下注射专利蓝（PB）、PB-LPS、空白脂质体（BL-LPS）或0.9%氯化钠溶液,检测谷丙转氨酶（ALT）、谷草转氨酶（AST）、尿素氮（BUN）、肌酐（Cr）,观察淋巴结染色情况.结果 注射PB-LPS、PB后区域淋巴结明显蓝染,两组的总体蓝染率分别是40%和20%（P〈0.05）,PB-LPS的淋巴标记效果明显优于PB.各组大鼠ALT、AST、BUN、Cr的差异均无统计学意义.结论 被动载药法制备的PB-LPS稳定性好、包封率高.

Objective To prepare patent-blue-lipesomes（PB-LPS）,a lymph-mapping developer.Methods PB was encapsulated in the liposomes using the passive loading method.The female Wistar rats were injected subcutaneously with the PB-LPS,PB,blank liposomes or isotonic rmtrium chloride at the heel solos,to study the stained situation of the lymph nodes and chemical analysis oftheir ALT,AST,BUN,Cr.Results The lymph nodes were obviously blue staining after injected PB-LPS or PB,the blue smimng ratios were 40%,20%（P〈0.05）,and the effect of PB-LPS on tracing of lymphy nodes was better than PB.No significant difference was found in ALT,AST,BUN and Cr.Conclusion The PB-LPS can be successfully prefabricated with such advantage as avirulence and high entrapment efficiency by passive loading method.