多菌灵降解菌的筛选及降解特性初步研究

Screening of carbendazim degrading bacterium and characteristic of carbendazim degradation

实验采用富集培养及划线纯化法,从多年生产多菌灵的原药厂下水道污泥中分离筛选到一株可有效降解多菌灵的细菌,经形态观察、生理生化实验及16S rDNA序列分析鉴定为红平红球菌属(Rhodococcus erythropolis)。该菌在温度30℃,pH6.0,补加葡萄糖、酵母浸粉做碳氮源的条件下,150 r/min摇床培养72 h,对多菌灵的降解率高达90.07%。用菠菜作盆栽试验,结果表明,L-1菌悬液可在6 d内有效降解多菌灵使其残留量达到国家相应标准,因而可以将其应用于多菌灵污染的生物修复。

A bacterium capable of degrading carbendazim effectively was isolated from sludge collected from sewer of a fungicide manufacturer.This bacterium was identified as Rhodococcus erythropolis by morphological observation,physiology biochemistry experiment and 16S rDNA gene sequence homologous analysis.L-1 strain could degradate carbendazim effectively and the degradating rate up to 90.07% after 72 h shaking culture with rotating speed 150 r/min,under the circumstances：30℃,pH 6.0,and add glucose,yeast powder for carbon and nitrogen source.Carbendazim in spinach could be effectively degraded by L-1 bacteria up to the national standard in 6 d.The experimental results showed that the strain was screened by experiment had certainapplication prospects.