金银藤挥发油成分的气-质联用分析与化学计量学解析

Analysis of Volatile Oil Constituents in Caulis Lonicerae by Gas Chromatography-mass Spectrometry and Chemometric Resolution Method

目的定性、定量分析金银藤挥发油成分。方法采用气相色谱-质谱进行检测,通过化学计量学解析法对二维色谱/质谱数据进行解析,得到各组分的纯色谱曲线和质谱,从而实现对金银藤挥发油成分的定性、定量分析,并结合程序升温保留指数来鉴定化合物。结果金银藤的挥发油鉴定了39个成分,定性组分含量占金银藤挥发油成分总含量的94.54%。结论金银藤挥发油的主要成分为十六烷酸(45.86%)、9,12-十八碳二烯酸乙酯(39.02%)、十四烷酸(1.8%)、十五烷酸(1.07%)、十八烷酸(1.02%)。

Objective Qualitative and quantitative analysis for the volatile oil constituents of Caulis Lonicerae（CL）.Methods Gas chromatography-mass spectrometry（GC-MS）,chemometric resolution method（CRM）,normalization methods and temperature-programmed retention indice（PTRI）were employed.Results39 volatile components of CL were determined,accounting for 94.54%total contenta of volatile oil of CL.Conclusion The main volatile components of CL are n-Hexadecanoic acid（45.86%）,9,12-Octadecadienoic acid,ethyl ester （39.02%）,Tetradecanoic acid（1.8%）,Pentadeccanoic acid（1.07%）and Octadecanoic acid（1.02%）.