解聚复肾宁对大鼠肾小球系膜细胞增殖和细胞周期的影响

Effect of Jiejufushenning on Proliferation and Cell Cycle of Rat Mesangial Cells in Virto

目的观察中药复方解聚复肾宁(JJFSN)对高糖环境下大鼠肾小球系膜细胞(mesangial cell,MC)增殖和细胞周期的影响。方法以高糖诱导MC增殖,采用血清药理学方法制备不同浓度JJFSN含药血清,加入培养液中,用MTT法检测细胞增殖,流式细胞技术检测细胞周期。结果 MTT显示:JJFSN含药血清可抑制高糖诱导的MC过度增殖(P<0.05),并且这种作用具有药物剂量和时间依赖关系。流式细胞技术分析表明:JJFSN可逆转高糖对细胞周期的影响,使G_0/G_1期细胞比例增加,S期细胞比例下降(P<0.05);在高浓度时还能促进MC细胞凋亡。结论JJFSN可以通过调节细胞周期,促进细胞凋亡,从而抑制高糖诱导的MC过度增殖,这可能是JJFSN防治DN早期的作用机理。

Objective：To investigate the effect of a traditional Chinese compound,Jiejufushening（JJFSN）on proliferation and cell cycle of rat mesangial cells.Methods：Mesangial cells were cultured under high glucose condition.Different concentrations of JJFSN pharmacological serum were prepared.Cell proliteration was assessed by the methylthiazoletetrazolium（MTr）assay.Cell cycle was detected by flow cytometry.Results：JJFSN pharmacological serum significantly（P〈0.05）inhibited MC proliferation under high ghcose condition,in a dose and timedependent manner by increasing the cell population in G0/G1 and reducing that in S-phase.Furthermore,high dose JJPSN pharmacological serum increased MC apoptosis.Conclusions：JJFSN Can reverse the influence of high glucose on MC proliferation through modulating cell cycle progress.It suggests that JJFSN could be a potential therapeutic agent for the prevention of DN in the early stage.