超低温保存对罗氏沼虾胚胎几种酶活性的影响

Effects of cryopreservation on enzyme activity in embryo of Macrobrachium rosenbergii

研究了超低温冷冻保存(-196℃)对罗氏沼虾胚胎内总三磷酸腺苷酶(ATPase)、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)等酶活性的影响。运用试剂盒分别测定了冷冻前后罗氏沼虾胚胎内酶活性的变化。结果表明,经过超低温冷冻保存后,胚胎内7种酶的活性均显著下降(P<0.05),其中总ATPase、CK、LDH和SDH的活性分别从冻前的1.322±0.162 U/mg prot、0.404±0.015 U/mg prot、352.225±23.214 U/gprot和2.067±0.139 U/mg prot下降到0.087±0.003 U/mg prot、0.010±0.002 U/mg prot、5.890±0.658 U/gprot和0.552±0.138 U/mg prot;SOD、CAT和GSH-Px的活性分别从冻前的19.217±0.677 U/mg prot、3.587±0.233 U/mg prot和7.626±1.106 U/(min.mg)下降至3.579±0.234 U/mg prot、1.773±0.227 U/mg prot和1.524±0.096 U/(min.mg);MDA的活性从1.015±0.038 n mol/mg prot上升到20.937±0.320 n mol/mgprot,超低温冷冻对罗氏沼虾胚胎酶活性有显著性影响。

Effects of cryopreservation（ -196℃ ） on the enzyme activity（ total ATPase, CK, SDH, LDH, SOD, CAT, GSH-Px and MDA） in the embryo of Macrobrachium rosenbergii were studied. The enzyme activity in the embryo was determined by reagent box. The results showed that the activity of seven kinds of enzymes dropped significantly after cryopreservation （P 〈 0.05 ）, and the activity of total ATPase, CK, LDH and SDH decreased from 1. 322 ±0. 162 U/rag prot, 0. 404 ± 0. 015 U/rag prot,352.225 ±23. 214 U/g prot and 2. 067 ±0. 139 U/rag prot to 0.087 ±0.003 U/rag prot, 0. 010 ±0. 002 U/rag prot,5. 890 ±0. 658 U/g prot and 0. 552 ± 0. 138 U/rag prot respectively; the activity of SOD, CAT and GSH-Px declined from 19. 217 ±0. 677 U/mg prot, 3. 587±0. 233 U/rag prot and 7. 626 ±1. 106 U/（ min . mg） to 3. 579±0. 234 U/rag prot, 1. 773±0. 227 U/rag prot and 1. 524 ± 0. 096 U/（ min.mg） respectively, but the activity of MDA increased from 1. 015±0. 038 n mol/mg prot to 20. 937 ±0. 320 n mol/mg prot. Cryopreservation had a great effect on the enzyme activity of embryo in Macrobrachium rosenbergii.