摘要
目的探讨汉防己甲素对成纤维细胞凋亡的调控作用。方法将汉防己甲素按质量浓度梯度(0.5,1.0,2.0mg/L)分组,并设空白对照组、乙醇对照组和阳性对照组(曲安缩松钠80mg/L),分别作用于原代培养的成纤维细胞,采用流式细胞仪检测各组细胞的凋亡率;以电泳法分离DNA片段,并以紫外光灯观察是否出现"梯状(ladder)"带。结果空白对照组细胞凋亡率为0;而2.0mg/L的汉防己甲素作用48h即出现典型的细胞凋亡峰,且具有质量浓度和时间依赖性,电泳后可见典型的200bp及其倍体片段。结论汉防己甲素能使成纤维细胞发生凋亡,且呈质量浓度和时间依赖性。
Objective To investigate the regulation effect of tetrandrine on fibroblast＇s apoptosis.Methods The experimental samples were divided into five groups,including blank control,ethanol control and positive control groups according to concentration gradient（0.5,1.0,2.0 mg/L）.Fibroblast＇s apoptosis index was detected by flow cytometry and DNA ladder was investigated by UV light after electrophoresis. Results T2 group was found typical apoptopic peak after treatment by 2.0 mg/L tetrandrine for 48 h and its apoptosis index rose with concentration and treatment time of tetrandrine.Its DNA after electrophoresis was found in UV light 200 bp and euploid pieces.But apoptosis index of blank control was zero.Conclusion Tetrandrine can induce apoptosis of fibroblast in quality concentration and time dependence.
出处
《中国药业》
CAS
2010年第11期8-9,共2页
China Pharmaceuticals
