石杉碱甲固体脂质纳米粒包封率的测定方法研究

Study on determination methods for entrapment efficiency of solid lipid nanoparticles loading huperzine A

目的建立石杉碱甲固体脂质纳米粒(Hup A-SLN)包封率的测定方法。方法分别采用透析法、超滤法分离固体脂质纳米粒和游离药物,以高效液相色谱法测定石杉碱甲的含量。结果超滤法能有效地将石杉碱甲固体脂质纳米粒与游离石杉碱甲分离,回收率为98.09%,测得3批样品的平均包封率为57.63%。结论超滤法方便快捷,结果准确,适用于石杉碱甲固体脂质纳米粒的包封率测定。

Objective To establish a method for the determination of the entrapment efficiency of solid lipid nanoparticles loading huperzine A（Hup A-SLN）.Methods The solid lipid nanoparticles and free drugs were separated by ultrafiltration and dialysis with HPLC for the assay of Hup A.Results Free drugs could be effectively separated from the Hup A-SLN by ultrafiltration.The average entrapment efficiency of three batches of Hup A-SLN was 57.63%,with the recovery of 98.09%.Conclusion It convenient,sensitive and accurate with ultrafiltration,and can be used to determine the entrapment efficiency of Hup A-SLN.