CpG ODN通过活化p38 MAPK上调A549细胞β防御素-2的表达

CpG ODN increases human beta-defensin 2 expression in A549 cell through p38 MAPK pathway

目的研究含CpG基序的寡核苷酸(unmethylated CpG motif containing oligodeoxynucleotide,CpG ODN)对人肺腺上皮细胞A549β防御素-2(humanβ-defensin 2,hBD-2)的表达及p38 MAPK磷酸化水平的影响。方法培养A549细胞,加或不加p38特异性抑制剂SB203580情况下用不同浓度CpG ODN进行干预。用RT-PCR法检测刺激后A549细胞hBD-2 mRNA表达变化,用Western Blot法检测细胞内p38MAPK蛋白磷酸化水平的变化。结果 CpG ODN刺激可以促进A549细胞内磷酸化p38 MAPK含量的增加,活化p38 MAPK途径,从而诱导hBD-2的表达,与对照组比较,P<0.05;用特异性阻断剂SB203580阻断p38 MAPK磷酸化可抑制CpG ODN的诱导作用。结论 CpG ODN通过p38 MAPK途径诱导hBD-2在呼吸道上皮细胞的表达,增强呼吸道对外界微生物的抵抗作用。

Objective To investigate the effects of unmethylated CpG motif containing oligodeoxynucleotide（CpG ODN） on the expression of human β-defensin 2（hBD-2） and the phosphorylation of p38 MAPK in lung epithelial cell line A549.Methods A549 cells were stimulated with CpG ODN at different concentrations in the presence or absence of SB203580,an inhibitor of p38 MAPK.The mRNA expression of hBD-2 was determined by RT-PCR and the phosphorylation level of p38 MAPK was detected by Western Blot.Results CpG OND significantly enhanced the expression of hBD-2 in A549 cells.The phosphorylation level of p38 MAPK was markedly up-regulated.CpG ODN-induced hBD-2 expression was suppressed by inhibition of p38 MAPK by SB203580.Conclusion CpG ODN might effectively up-regulate hBD-2 expression in lung epithelial cells through p38 MAPK pathway.