注意缺陷多动障碍患儿胃泌素释放肽受体基因多态性的研究

Study of the GRPR polymorphisms in ADHD children

目的了解胃泌素释放肽受体(GRPR)在注意缺陷多动障碍(ADHD)中的多态性表现,分析GRPR第二外显子的基因序列结果。方法 2005—2007年西安市儿童医院的120例ADHD初诊患儿和126例正常儿童,用红细胞裂解法提取外周静脉血DNA,通过PCR扩增并测序得到GRPR第2外显子基因序列。分别观察ADHD组、正常对照组间和多动/冲动、注意力障碍、混合各表型间基因频率和基因型频率是否存在差异。结果 GRPR第二外显子450和661位点存在TT、TC和CC3种多态性;正常儿童组和ADHD组间GRPR基因第二外显子基因频率和基因型频率差异无统计学意义(χ2=0.30、0.52、1.34、0.30,P均>0.05);多动/冲动、注意力障碍及混合各型间基因频率、基因型频率差异无统计学意义(χ2=0.37、0.49、0.63,P均>0.05)。结论未得出GRPR基因第二外显子多态性与ADHD患儿存在相关性的依据。

Objective To study the GRPR polymorphisms in ADHD children and analyze the sequence of the second exon of GRPR. Methods The DNA was taken from the periphery blood. The PCR of the second exon of 120 children with ADHD and 126 normal children was sequenced. We used Chi-square test to know difference of genotype and haplotype between ADHD and normal control group, and among inattention, hyperactivity and impulsivity compound group. Result There were TT, CC and TC polymorphisms in the second exon of GRPR in 661 and 450 sits. There was no difference between ADHD and normal control group （ Х^2 = 0.30, 0.52, 1.34, 0.30; all P 〉 0.05）. There was no difference among inattention, hyperactivity and impulsivity compound group （Х^2= 0.37, 0.49, 0.63, all P 〉 0.05）. Conclusion The research has not proved the relationship between ADHD and the second exon of GRPR.