淋病奈瑟菌PCR检测的临床应用研究

Clinical Application of PCR Assay for Detection of Neisseria gonorrhoeae

为评价淋病奈瑟菌ＰＣＲ检测的临床效果，建立了对标准淋病奈瑟菌的ＤＮＡＰＣＲ检测技术。结果显示，ＮＧＰＣＲ技术的灵敏度约为３个菌的基因组拷贝。对８种菌株进行ＮＧＰＣＲ，只有标准淋病奈瑟菌显示阳性，其余７株非淋球菌菌株均阴性，说明有很好的特异性。对４９例淋病患者同时进行涂片镜检法、细菌培养法和ＰＣＲ法检测的比较，其阳性检出率分别为７６．６％、５２．２％和９５．９％。提示ＮＧＰＣＲ方法比涂片和培养法更为敏感，且特异性强，在淋病的诊断，特别是早期诊断中有重要参考价值。

Inder to evaluate the clinical application of PCR assay for the detection of Neisseria gonorrhoeae . Genitourinary specimens were obtained from NG patients and Neisseria gonorrhoeae was detected with a number of tests including Gram′s stain of smears, culture and PCR assay. The sensitivity and specificity of the NG PCR assay were evaluated with 8 bacterial strains. The NG PCR assay had a detection limit of 3 copies of NG genomic DNA. In the specificity of the PCR assay, the standard Neisseria gonorrheae was positive, but no PCR product could be visualized in 7 nongonorrhoeae strains. PCR results of 47 NG patients were compared with those obtained by Gram′s smear and culture. The positive rates for smear, culture and PCR were 76.6%, 52.2% and 95.9%, respectively. Of 82 normal controls, 1 specimen was PCR positive. The false positive rate of the PCR assay was about 1.2%. The PCR assay is a satisfactory diagnostic tool for the detection of Neisseria gonorrhoeae in clinical specimens.