N-乙酰半胱氨酸对肠系膜上动脉夹闭后肠和肺损伤的防治效应

The Protective Effect of N-acetylcysteine oil Intestine and Lung Damage Induced by Superior Mesenteric Artery Occlusion

Ｎ－乙酰半胱氨酸（ＮＡＣ）是体内游离巯基的组成部分，既可补充体内谷胱甘肽，又具有清除自由基、保护细胞的作用。本实验观察了ＮＡＣ在大鼠肠系膜上动脉夹闭（ＳＭＡＯ）造成的肠缺血再灌损伤和继发性肺损伤时对肠、肺的保护效应。ＳＭＡＯ４５ｍｉｎ松夹，于松夫前、后５分钟尾静脉注射ＮＡＣ（５０ｍｇ／ｋｇ），测定松夹后２、４、６ｈ肠、肺组织总巯基（ＴＳＨ）和游离巯基（ＮＰＳＨ）以及丙二醛（Ｍ ＤＡ）和髓过氧化物酶（Ｍ ＰＯ）的改变。结果显示静注ＮＡＣ后可明显增加肠肺ＮＰＳＮ．降低肠、肺ＭＤＡ和ＭＰＯ，说明ＮＡＣ对缺血再灌注引起的肠、肺损伤有防治效应。

N-acetylcysteine (NAC)is a main part of free thiol in vivo,a precursor of glutathione. and has some effects on scavenging free radicals and protecting cells. The goal of this study was to observe the effect of NAC on intertinal ischenlia-reperfused injury and lung secondary damage in the model of superior mesenteric artery occlusion (SMAO)in rats. After SMAO 45 minutes. unocclusion was performed, NAS (50mg /kg) was ijnjected through tail vein at 5min pre-reperfusion and post - repcrfusion. The tissues of intestine and lung were sampled at 2, 4 and 6h after unocclusion for determination of totalTSH. free thioINPSH,malondialdehyde (MDA)and activity of myeloperoxidase (MPO). The results showed that intestinal and lung tissues NPSH increased and MDA and MPO activities decreased aften i. v. NAC given post-reperfusion. It is Suggested that NAC possess protective or treating affect on intestinal ischenlia-reperfused injury and lung secondary damage.