粉防己碱对人翼状胬肉成纤维细胞体外增殖及增殖细胞核抗原表达的影响

Effects of tetrandrine on proliferation and expression of PCNA of human pterygium fibroblasts

目的:观察粉防己碱(Tet)对体外培养人翼状胬肉成纤维细胞(HPF)增殖的影响。方法:用20、40、80和160μmol/L的Tet作用于体外培养的HPF,24、48及72h后采用MTT法检测细胞生长抑制率,免疫细胞化学法检测HPF增殖细胞核抗原(PCNA)的表达情况。结果:Tet在20、40、80和160μmol/L浓度下作用24、48及72h,能够抑制HPF增殖(F时间=235.632、342.039、586.788和584.667,P均<0.001;F组间=372.672、474.734和389.467,P均<0.001)。Tet干预后PCNA蛋白表达下降(F=185.623,P=0.009)。结论:Tet可抑制翼状胬肉HPF细胞增殖。

Aim：To investigate the effects of tetrandrine on proliferation of human pterygium fibroblast （HPF） in vitro.Methods：Different concentrations （20,40,80,and 160 μmol/L） of Tet were added into cultured HPF for 24,48,and 72 hours. The MTT method was used to assay the biologic activities of Tet.The expression of proliferating cell nuclear antigen（PCNA） in each group was detected by using immunocytochemistry method.Results：In 20,40,80,and 160 μmol/L concentration group,Tet could inhibit the proliferation of HPF（Ftime=235.632,342.039,586.788 and 584.667,P0.001;Fgroup=372.672,474.734 and 389.467,P0.001）. After the intervention of Tet,the PCNA protein expression was dropped （F=185.623,P=0.009）.Conclusion：Tet could inhibit the proliferation of HPF.