胃癌组织中Beclin 1和PTEN蛋白的表达

Expression of Beclin 1 and PTEN proteins in gastric cancer tissue

目的:检测胃癌组织中自噬相关基因Beclin 1和PTEN蛋白的表达情况。方法:采用免疫组化SP法检测62例胃癌组织和36例正常胃组织中Beclin 1和PTEN蛋白的表达。结果:胃癌组织中Beclin 1和PTEN蛋白的阳性表达率分别为40.0%(25/62)和45.2%(28/62),均低于正常对照组的94.4%(34/36)和94.4%(34/36)(χ2分别为27.845、23.800,P均<0.001);胃癌组织分化程度越低,Beclin 1蛋白的阳性表达率越低(χ2=26.230,P<0.001);组织浸润深度越深、分化程度越低、伴淋巴结转移的胃癌组织PTEN阳性表达率越低(χ2=10.020、23.296和11.610,P均<0.001)。胃癌组织中Beclin 1和PTEN的表达呈正关联(rP=0.578,P<0.001)。结论:自噬活性的改变可能与胃癌的发生发展有关。

Aim：To investigate the expressions of autophagy-related gene Beclin 1 and PTEN in gastric cancer tissue.Methods：Immunhistochemistry was used to detect the expressions of Beclin 1 and PTEN in 62 cases of gastric cancer and 36 cases of gastric normal tissue.Results：The positive expression rates of Beclin 1 and PTEN in normal tissues were 94.4% and 94.4%,significantly higher than those in cancer tissue （40.0% and 45.2%,χ2=27.845,23.800,respectively,P0.001）. The expression of Beclin 1 in gastric cancer tissue was associated with cancer differentiation（χ2=26.230,P0.001）. The expression of PTEN in gastric cancer tissue was related with cancer invasive extent,differentiation degree and lymph node metastasis（χ2=10.020,23.296 and 11.610,respectively,P0.001）. The expression of Beclin 1 in gastric cancer was related to the expression of PTEN（rP=0.578,P0.001）.Conclusion：The change of autophagic capacity may be related to carcinogenesis and the development of gastric cancer.