苯丁酸钠体外对YTMLC-90细胞生长的影响

Inhibitory effects of sodium phenylbutyrate on YTMLC-90 cells in vitro

目的:观察苯丁酸钠(PB)体外对人个旧肺鳞状细胞癌YTMLC-90细胞生长的影响。方法:YTMLC-90细胞分为6组,对照组不作任何处理,余5组分别给予250、500、1000、2000和4000mg/LPB,分别作用48、72和96h。采用MTT法观察各组细胞生长情况,计算细胞生长抑制率。另取YTMLC-90细胞分为3组:对照组、500和1000mg/LPB组,分别作用48和72h,采用流式细胞术检测3组细胞的细胞周期及凋亡情况。结果:不同质量浓度的PB作用48、72和96h,各组细胞的生长抑制率相比,差异均有统计学意义(F=672.413、1633.321和1196.548,P均<0.05)。PB作用48和72h后,各组细胞G0/G1期、S期与G2/M期细胞百分率及凋亡率比较,差异均有统计学意义(F48h=508.347、224.522、19.890和111.849,F72h=311.979、102.923、247.432和177.018,P均<0.05)。与对照组相比,PB作用后YTMLC-90细胞生长缓慢,细胞阻滞于G0/G1期,凋亡增加(P<0.05)。结论:PB可抑制YTMLC-90细胞的生长,使其阻滞于G0/G1期,凋亡增加。

Aim：To study the effects of sodium phenylbutyrate（PB） on human lung squamous cancer cell line YTMLC-90.Methods：YTMLC-90 cells were allocated into 6 groups. The control group was not treated,and the other 5 groups were given 250,500,1 000,2 000,and 4 000 mg/L PB,and treated for 48,72,and 96 h.MTT assay was used to evaluate the inhibitory effects of PB on the cells.YTMLC-90 cells were allocated into 3 groups.The control group was not treated,and the other 2 groups were given 500 and 1 000 mg/L PB for 48 and 72 h,respectively. The flow cytometry was applied to detect the apoptotic rate and alteration of cell cycle.Results：After being treated for 48,72,and 96 h,the cell growth inhibition rate among the 6 groups had significant differences （F=672.413,1 633.321,and 1 196.548,all P0.05）.After being treated for 48 h,the fraction of G0/G1 phase cells,S phase cells,and G2/M phase cells,and the apoptosis rate among the 3 groups had significant differences （F48 h=508.347,224.522,19.890,and 111.849;F72 h=311.979,102.923,247.432,and 177.018,all P0.05）. PB could inhibit the growth of YTMLC-90 cells and most of the cells were arrested at G0/G1 phase（P0.05）.Conclusion：PB could inhibit the growth of YTMLC-90 cells in vitro. The inhibitory effects of PB on YTMLC may through the increasing of G0/G1 fraction and apoptosis induction.