5-氟尿嘧啶固体脂质纳米粒在小鼠体内的分布

Distribution of 5-fluorouracil solid lipid nanoparticles in mice

目的:观察5-氟尿嘧啶固体脂质纳米粒(5-FU-SLNs)在小鼠体内的分布特征。方法:取42只小鼠分为2组,分别以60mg/kg的剂量尾静脉注射5-FU-SLNs和游离5-FU,于给药后不同时间采用高效液相色谱法测定心、肝、胃、肺和肾等组织中5-FU的浓度,对5-FU-SLNs在小鼠体内组织的分布特征进行评价。结果:与游离5-FU相比,5-FU-SLNs在肾和肺中的总靶向效率分别从(20.42±0.08)%和(21.29±0.23)%增至(34.34±0.02)%和(23.29±0.03)%(t=21.760和11.656,P均<0.05),在心、肝和胃中的分布无明显变化(t=8.043,36.097和7.457,P>0.05)。结论:5-FU-SLNs可改变小鼠体内药物的分布,对肺和肾具有亲和性和靶向作用。

Aim：To study in vivo biodistribution of 5-fluorouracil solid lipid nanoparticles（5-FU-SLNs） in mice.Methods：HPLC method was established to determine 5-FU in mice tissue homogenates.A total of 42 mice were randomly allocated into two groups.5-FU-SLNs and 5-FU were administered by tail vein at dose of 60 mg/kg,respectively and tissues were excised and handled properly at different time points to quantify 5-FU by HPLC. The biodistribution characterization of 5-FU-SLNs was evaluated.Results：The contents of 5-FU in the kidney and lung were significantly higher in 5-FU-SLNs group than those in 5-FU group [（34.34±0.02）% vs. （20.42±0.08）%,（23.29±0.03）% vs. （21.29±0.23）%]（t=21.760 and 11.650,P0.05）.Conclusion：5-FU-SLNs have affinity and are targetting to lung and kidney in mice.