糖蛋白在胆囊良恶性肿瘤中的表达

Expression of P glycoprotein in benign and malignant gallbladder neoplasms

目的检测Ｐ糖蛋白在不同胆囊组织中的表达，以探讨该蛋白与胆囊癌抗化疗特性的关系及作为胆囊癌生物标志物的潜在价值。方法采用碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）的免疫组化方法，用二种决定簇不同的抗人Ｐ糖蛋白的单克隆抗体ＪＳＢ１和ＵＩＣ２，检测２６例胆囊癌、１４例胆囊良性肿瘤及９例正常胆囊组织中Ｐ糖蛋白的表达情况，并分析其表达程度与不同临床资料的相关关系。结果单抗ＪＳＢ１测得Ｐ糖蛋白在胆囊癌中表达的阳性率为７６．９％，显著高于胆囊良性肿瘤组及正常胆囊组（阳性率分别为３５．７％和３３．３％，Ｐ＜０．０５）。单抗ＵＩＣ２测得Ｐ糖蛋白在胆囊癌中表达的阳性率为６９．２％，也显著高于胆囊良性肿瘤组及正常胆囊组（阳性率分别为２１．４％和１１．１％，Ｐ＜０．０１）。Ｐ糖蛋白的表达阳性率与胆囊癌的ＴＮＭ分期及胆囊良恶性肿瘤的病理类型均无相关关系。结论Ｐ糖蛋白在胆囊恶性肿瘤中的过表达，提示其在胆囊癌对化疗不敏感的特性中起着重要作用，同时Ｐ糖蛋白在不同胆囊组织中表达的显著差异性。

Objective To evaluate the relationship between P glycoprotein expression and anti cancer drug resistance of gallbladder carcinoma and the use of P glycoprotein as a biomarker of gallbladder carcinoma, the expression of P glycoprotein was detected in benign and malignant gallbladder neoplasms and normal gallbladder tissues. Methods Alkaline phosphatase anti alkaline phosphatase ( APAAP ) method was used to detect the expression of P glycoprotein in different gallbladder tissues ( gallbladder carcinoma, 26 cases; benign gallbladder neoplasm, 14 cases; and normal gallbladder tissue, 9 cases). The relationship between expression of P glycoprotein, TNM stages and other clinical data of gallbladder carcinoma was also analyzed. Results Immunohistochemical staining with a monoclonal antibody JSB 1, P glycoprotein was positive in 76.9% (20/26) of gallbladder carcinomas, in 35.7% (5/14) of benign gallbladder neoplasms and in 33.3% (3/9) of normal gallbladder tissues ( P <0.05). With another monoclonal antibody UIC 2, the positive rates were 69.2% (18/26), 21.4% (3/14) and 11.1% (1/9), respectively ( P <0.01). There was no significant correlation between P glycoprotein expression and gallbladder carcinoma TNM staging. Conclusion The results suggsest that P glycoprotein probably plays an important role in the poor response of gallbladdar carcinoma to chemotherapeutic agents. In addition, P glycoprotein may be used as a valuable biomarker of gallbladder carcinoma.