幽门螺杆菌相关性胃疾病患者末梢血中Hp-CagAIgG的检测

Detection of Hp-CagA IgG In blood ending in Hp-infected gastroduodenal diseases.

目的:了解细胞毒素相关蛋白Ａ在Ｈｐ相关性胃疾病中的检出情况，并对所采用的方法进行评价。方法采用斑点金免疫渗滤试验（ＤＩＧＦＡ），对１６８例胃粘膜活检组织快速尿素酶试验、ＰＣＲ检测及Ｗａｒｔｈｉｎ－Ｓｔａｒｒｙ染色任二项Ｈｐ阳性者的末梢血进行Ｈｐ－ＣａｇＡＩｇＧ检测。结果１６８例Ｈｐ阳性者中，１４２例Ｈｐ－ＣａｇＡＩｇＧ阳性（８４．５２％），其中慢性浅表性胃炎６５．６３％（２１／３２），慢性萎缩性胃炎７８．５７％（３３／４２），十二指肠球部溃疡９４．７４％（３６／３８），胃溃疡９１．６７％（３３／３６），胃癌９５％（１９／２０）。结论ＤＩＧＦＡ能够快速诊断ＣａｇＡ阳性Ｈｐ感染，与ＥＬＩＳＡ法相比除具有较高的特异性及敏感性外，还具有简便、快速、准确等特点。

Objective To investigate the prevalence of cytotoxin associated gene A (CagA) inHp-infected gastroduodenal diseases and to evaluate the methods used. Methods 168 Hp-infectedpatients were examined to see if they have Hp-CagA IgG in their peripherat blood by dot immunogold filtration assay (DIGFA) who were examed by rapid urase, PCR and Warthin-Starry stain andtwo of these three examinations were positive. Results 142 in 168 patients were detected to haveHp-CagA IgG in their peripheral blood (85. 52%), including chronic superficial gastritis 65. 63%(21/32), chronic atrophic gastritis78. 57% (33/42), duodenal ulcer94. 74% (36/38), gastric ulcer91. 67% (33/36) and gastric cancer95. 00% (19/20). Conclusion DIGFA may be used to detectCagA-Hp infection, not only shows better sensitivity and specificity, but also shows convenient,rapid and reliable features compared with ELISA.