胃粘膜上皮异型增生和胃癌组织CD_(44)V_6表达的免疫组化研究

Expression of CD 44 Splice Variant V 6 in Human Dysplastic and Carcinomatous Gastric Mucosa

为了探讨ＣＤ４４Ｖ６表达与胃癌发生发展的关系，采用ＳＰ免疫组化染色方法，检测２０例正常胃粘膜上皮，４３例异型增生和８５例胃癌组织ＣＤ４４Ｖ６的表达情况。结果显示，正常胃粘膜无ＣＤ４４Ｖ６表达；异型增生ＣＤ４４Ｖ６阳性表达率为３０．２％（１３／４３），其中Ⅰ级、Ⅱ级、Ⅲ级异型增生阳性率分别为１３．６％（３／２２）、３３．３％（４／１２）和６６．７％（６／９），提示ＣＤ４４Ｖ６表达阳性率与异型增生严重程度密切相关（Ｐ＜０．０５）；胃癌组织阳性表达率为７４．１％（６３／８５），其表达阳性率和染色强度均高于异型增生，Ⅰ期、Ⅱ期胃癌ＣＤ４４Ｖ６染色强度和阳性表达率均显著低于Ⅲ期和Ⅳ期（Ｐ＜０．０５）。结果表明ＣＤ４４Ｖ６表达与胃癌发生发展密切相关，并发生于癌变的早期阶段。

To evaluate the relationship between CD 44 splice variant V 6(CD 44 V 6) expression and carcinogenesis and progression of gastric cancer.Methods the expression of CD 44 V 6 protein was detected immunohistochemically in 148 resected gastric specimens Among them,20 cases were normal,43 cases were dysplastic and 85 cases were carcinomatous pathologically.The normal gastric mucosa did not reveal expression of CD 44 V 6,whereas the positive rate of CD 44 V 6 immunoreactivity was 30.2%(13/43) in dysplastic mucosa.There was significant correlation between the grading of dysplasia and the CD 44 V 6 positive rate( P <0.05).The positive rate in cancer was 74.1%(63/85),it was significantly higher than that of dysplasia;The CD 44 V 6 positive rate and staining intensity of the tumor in stage Ⅰ and Ⅱ were significantly lower than that in stage Ⅲ and Ⅳ( P <0.05).Generation of CD 44 V 6 may be linked with gastric carcinogenesis and progeression.The up regulated expression of CD 44 V 6 may take place during early stage of gastric carcinoma,determination of CD 44 V 6 protein may be useful in the diagnosis of early gastric cancer.