膀胱癌患者细胞粘附分子水平的临床意义

Serum level of cell adhesion molecule in bladder neoplasms

目的探讨细胞间粘附分子（ＩＣＡＭ１）、血管细胞粘附分子（ＶＣＡＭ１）与膀胱癌生物学行为的关系。方法采用ＥＬＩＳＡ方法检测２６例膀胱癌与１０例正常人的血清ＩＣＡＭ１、ＶＣＡＭ１。采用免疫组化技术检测ＩＣＡＭ１在膀胱癌组织和正常膀胱粘膜中的表达。结果膀胱癌组术前血清ＩＣＡＭ１、ＶＣＡＭ１分别为３０４１μｇ／ｍｌ和１１３３６μｇ／ｍｌ，明显高于对照组１８８１μｇ／ｍｌ和４９６９μｇ／ｍｌ，其含量随肿瘤分级、临床分期递增而升高。膀胱癌组１４例术后３个月、６个月随访，血清ＩＣＡＭ１、ＶＣＡＭ１较术前明显降低。初发与复发膀胱癌血清ＩＣＡＭ１、ＶＣＡＭ１均升高，但两者差异无显著性。膀胱癌组织１０例中ＩＣＡＭ１阳性表达６例。结论血清ＩＣＡＭ１、ＶＣＡＭ１水平对膀胱癌早期诊断，肿瘤分级，临床分期。

Objective To clarify the correlation beween serum intercellular adhesion molecule 1 (ICAM 1)、 vascular cell adhesion molecule 1 (VCAM 1) and bladder neoplasm. Methods Serum ICAM 1 and VCAM 1 were detected by means of ELISA technique in 26 cases of bladder transitional cell carcinoma.10 cases of nontumor urogenital diseases or normal subjects as controls. Results The mean contents of ICAM 1、VCAM 1 were significantly higher in bladder cancer patients (304.1μg/ml、 1 133.6 μg/ml) than in the controls (188.1μg/ml、496.9μg/ml),These were elevated with the advance of tomor grade and tomor stage.Three and six months after operation,both the ICAM 1 and VCAM 1 were significantly reduced,while in the relapse cases,the ICAM 1 and VCAM 1 would rise again.Immunochemical technique revealed that the rise of serum ICAM 1 content is related to the increased expression of neoplastic tissue. Conclusions Serum ICAM 1 and VCAM 1 levels were claimed to be valuable markers in the early diagnosis and prognosis of bladder cancer and is valuable in the follow up study of bladder cancer.