气相色谱法测定活络膏中薄荷脑和水杨酸甲酯的含量

Determination of the contents of mentholum and methyl salicylate in Medi-cated Balm by capillary gas chromatography

目的:建立活络膏中有效成分薄荷脑和水杨酸甲酯的毛细管气相色谱测定方法。方法:色谱条件以PEG-20M毛细管柱30m×0.25mm×0.25μm,程序升温,起始温度为80℃,保持5min后每分钟升高15℃,终止温度为190℃,保持8min,分流比50∶1。结果:各组分在相应的浓度呈良好线性关系,r1=0.99979、r2=0.99998,RSD≤2.0%。结论:本方法简便、快速、准确,可用于活络膏的质量控制。

Objective：To establish a method for determination of the mentholum and methyl salicylate in Medicated Balm by capillary gas chromatography.Methods：Chromatographic condition was PEG-20M capillary column 30 m ×0.25 mm× 0.25 μm,initial temperature was 80℃,temperature programmed to raise 15℃ in five minutes,final temperature was 190℃ and kept eight minutes,split ratio was 50：1.Results：There was a favorable linearity relation between peak area and concentration.r1=0.999 79,r2=0.999 98,RSD≤2.0%.Conclusion：The method is sensitive,accurate and can be used for quality control of Medicated Balm.