药用黄芪的遗传多样性RAPD分析

Genetic Diversity Analysis of Scutellaria baicalensis by RAPD

目的:对药用黄芪的遗传多样性进行研究,为黄芪的保护利用提供参考。方法:采用随机扩增多态DNA(RAPD)方法对15份黄芪基因组进行扩增,所获数据通过NTSYS-pc v2.1和POPGENE v1.3进行分析。结果:从47条引物中筛选出12条条带清晰且重复性好的引物用于实验和统计分析,共扩增出85个位点,多态性位点占78.16%。聚类分析显示,所有供试黄芪可明显聚为三类。结论:本研究中来自四个省份的黄芪具有较高的遗传多样性;材料间遗传距离的远近与其地理来源有一定相关性,其中四川理塘的野生黄芪与其他材料遗传距离较大,推测与其独特的生境有关。说明保护黄芪多样性应尽可能广泛收集不同来源地的种质资源。

Objective： To have well knowledge of the genetic diversity of Scutellaria baiculensis. Methods： 12 random primers were selected to study on 15 S. baicalensis from four provinces by RAPD. The application result was record with 0 or 1 and the data was calculated with NTSYS -PC v2. 1 and POPGENE vl. 31. Results： The selected 12 random primers used in PCR amplification to detected 85 bands, among which 78. 16% percent was polymorphic. The results of cluster ananlysis show that all the- accessions researched in this study were classified into three categories. Conclusions： The genetic variation was relatively high in S. baicalensis, and the genetic similarity among the accesions is related with the geographic origination. Therefore, to protect the S. baicalensis germplasm should assemble accessions from different areas as extensively as possibly.