低剂量三丁基锡暴露对小鼠睾酮水平的影响

Effect of Exposure to Low-dose Tributyltin Chloride on Serum Testosterone Levels in Mice

目的探讨低剂量氯化三丁基锡(TBTCl)暴露对小鼠睾酮水平的影响。方法将36只21日龄SPF级雄性昆明小鼠随机分为0.5、0.05mg/kg TBTCl染毒组和对照组(玉米油),每组12只。从出生后第24天(PND24)起,采用腹腔注射方式染毒,每3天1次,染毒至PND45,期间观察包皮分离时间。小鼠继续喂养至PND56,检查其PNDs49、56时血清睾酮水平,在PND56称取肝脏、肾脏、胸腺、脾脏、睾丸、附睾重量,并计算脏器系数。结果各组小鼠体重、肝脏、肾脏、胸腺、脾脏、睾丸、附睾重量及其脏器系数以及包皮分离时间比较,差异均无统计学意义。PND49时,0.5mg/kg TBTCl染毒组小鼠血清睾酮水平低于对照组,差异有统计学意义(P<0.01),但在PND56时恢复正常。结论在未产生明显毒性作用的剂量下,TBTCl染毒可导致暴露小鼠血清睾酮浓度水平降低,但这种干扰作用是可逆的。

Objective To explore the effects of exposure to low close tributyhin chloride （TBTC1） on serum testosterone （T） levels in mice. Methods Thirty-six SPF male KM mice aged 21 days were randomly divided into 3 groups by body weight, 12 in each, and treated with TBTC1 at the doses of 0.5,0.05 mg/kg or vehicle （corn oil） by intraperitoneal injection, every 3 days from postnatal days （PNDs） 24 to 45. The age of mice was recorded on the day that preputial separation was achieved during exposure period. The mice were kept until PND 56, whose serum T levels were detected on PNDs 49 and 56.The organ weights were measured and counted corresponding organ index on PND 56. Results The difference among all the groups was not significant on body gain, organ weights （liver, kidney, thymus, spleen, testes, and epididymis） and their organ index, and the age of preputial separation. Serum T levels significantly decreased at PND 49 in 0.5 mg/kg TBTC1 group compared with the control （P〈0.01）, and then followed by an obvious recovery at PND 56. Conclusion Exposure to non-toxic dose of TBTC1 may reduce serum T levels of KM mice, however, this inhibitory effect is reversible.