对羟基苯甲酸丙酯对人角质形成细胞毒作用研究

Cytotoxicity of n-propylparaben to HaCat Keratinocytes in Culture

目的探讨对羟基苯甲酸丙酯对人角质形成细胞(HaCat)的细胞毒作用。方法选用人角质形成细胞株,分别观察不同剂量(3.125～200μg/ml)对羟基苯甲酸丙酯作用不同时间(24～72h)对其细胞毒作用。通过MTT比色法测定其对细胞增殖活力的影响;乳酸脱氢酶(LDH)释放法测定其对细胞膜的损伤。结果对羟基苯甲酸丙酯可引起培养细胞数量明显减少,随作用时间的延长,剂量的增加,对角质形成细胞增殖的抑制作用也显著增大,作用24h在50μg/ml出现细胞增殖抑制,作用48h在25μg/ml出现细胞增殖抑制,而作用72h在6.25μg/ml时就出现细胞增殖抑制,呈现明显的时间-剂量-效应关系;与对照组比较,从50μg/ml起,LDH释放率明显增加,并随剂量的增加而呈上升趋势。结论对羟基苯甲酸丙酯对HaCat细胞具有明显的细胞毒作用,呈现明显的时间-剂量-效应关系。

bjective To know the cytotoxieity of n-propylparaben to HaCat keratinocytes in vitro. Methods HaCat keratinoeytes were exposed to n-propylparaben at different doses（ 3.125-200 μg/ml ） and for different time（24-72 h ）. The effect of n-propylparaben on the proliferation of HaCat keratinocytes was evaluated by MTT assay. Lactate dehydrogenase （LDH） level was detected. Results The decreasing cell viability was observed in n-propylparaben exposure groups at 50 μ/ml for 24 hours, at 25 μg/ml for 48 hours and at 6.25 μ/ml for 72 hours with a dose- time-effect dependent manner. Th~ increasing release rates of LDH were observed among the n-propylparaben exposure groups at the doses of more than 50 μg/ml with a dose-effect dependent manner. Conclusion n-propylparaben can induce cytotoxicity to HaCat keratinocytes in vitro.