蔬菜中农药多组分残留量的固相萃取-气相色谱测定法

Determination of Pesticide Residues in Vegetables by Solid Phase Extraction and Gas Chromatography

目的建立蔬菜中农药固相萃取-气相色谱测定法。方法蔬菜样品经过粉碎、浸泡用固相萃取法提取,提取液采用FPD检测有机磷、用ECD检测有机氯及菊酯类农药。结果有机磷、有机氯、拟除虫菊酯线性范围,相关系数分别为0.125～6.25μg/ml,r=0.9991～0.9998;0.005～1.0μg/ml,r=0.9993～0.9996;0.010～2.0μg/ml,r=0.9989～0.9998,相对标准偏差为0.82%～2.5%,加标回收率为88.5%～110.0%。结论固相萃取法前处理结合气相色谱法测定蔬菜中农药多残留含量操作简单、快捷,去除蔬菜基体对农药残留检测带来的严重干扰,具有可靠的准确度和良好的精密度。

Objective To establish a method for the determination of pesticide residues in vegetables by solid phase extraction and gas chromatography. Methods The samples were ground, soaked, and extracted by solid phase, organophosphoros pesticide residues were determined by GC with FPD detector and organochlorine and pyrethroid pesticides compounds by GC with ECD detector. Results The linearity range and coefficients of correlation were 0.125-6.25 g/ml and 0.999 1 -0.999 8 for organophosphorus respectively,and 0.005-1.0 g/ml and 0.999 3-0.999 6 for organochlorine,and 0.010-2.0 g/ml and 0.998 9- 0.999 8 for pyrethroid. The relative standard deviation varied between 0.82% to 2.5%, and recovery rate ranged from 88.5% to 110.0%. No significant difference was found between this method and national standard method. Conclusion The method has the advantages of easy and quick operation, more disturbances being eliminated and higher accuracy and precision.