加速溶剂萃取仪提取菜粕芥子碱工艺研究

The Study of Rapeseed-Meal-Sinapine Extraction by Solvent Extrator

采用单因素试验和L9(34)正交试验设计优化菜粕芥子碱的加速溶剂萃取仪萃取工艺条件。确定了影响菜粕芥子碱得率的主次因素为:上样量、萃取次数、萃取温度和萃取时间,最佳萃取工艺条件为:萃取温度为140℃,静态萃取时间为15 min,萃取次数为3次,上样量为3 g;在最佳工艺条件下,菜粕芥子碱的得率为1.8807%,与热醇回流提取试验相比,得率提高了64.526 3%,经ASE萃取后的菜粕品质也有较大提高,通过红外光谱对提取浓缩液冷冻干燥后的样品分析,证实样品中含有芥子碱特征结构。

Single-factor test and L9 （34） orthogonal experimental design were used in the study of Rapeseed-meal- sinapine extraction. Sinapine was measured at 388nm spectrometry, OZT was measured by ISO 5504-1983, TITTO was measured by the total phenols , and protein was determined by Kjeldahl＇ s method. Study showed that the primary and secondary factors were： the sample quality, cycles, extraction temperature and extraction time; The optimum con- ditions were： extraction temperature 140 ℃, static extraction time 15min, 3 times extraction, sample quantity 3g. Under the optimum conditions, the dregs of Sinapine yield was 1. 8807 % , which was 64. 5263% increasing compared to the hot alcohol extraction. The quality of rapeseed meal after ASE extracted was improved. By infrared spectroscopy analysis, it verified that the eigenstructure of sinapine was existed in concentrated freeze-dried extracts.