摘要
经热水提取、醇沉、大孔树脂分离、三氯乙酸脱蛋白、H2O2脱色、透析、DEAE-52纤维素和SephadexG-200凝胶柱层析,建立了白花蛇舌草多糖的分离纯化方法。制备的ODⅠ经紫外扫描及HPGPC测试,证实为单一纯品。ODⅠ经PMP柱前衍生高效液相法测定,确定其由鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖组成。
A method of preparation of polysaccharide from Oldenlandia diffusa wild was identified, using hot water extract method, ethanol precipitation, removing the proteins by TCA reagent, decoloring with hydrogen peroxide and dialyzing by distilled water. After that, one of polysaccharides was isolated and purified through DEAE -52 and SephadexG -200 chromatography, which was named OD I. The purity of OD I was examined by UV and HPCPC. The composition of monosaccharides of OD I was mannose, glucose, galactose, xylose and arabinose which was determined by HPLC with pre -column derivatization.
出处
《云南中医学院学报》
2010年第3期43-46,共4页
Journal of Yunnan University of Traditional Chinese Medicine
