Th17细胞在脓毒症中的作用机制研究

Role of Th17 cells in pathogenesis of sepsis

目的探讨脓毒症患者外周血辅助性T细胞(THelper,Th)17的水平及意义。方法将2009年3-7月在我院急诊与外科ICU住院的脓毒症患者55例按照疾病严重程度分为3组:脓毒症组18例,重症脓毒症组29例,脓毒性休克组8例。四色流式细胞术检测CD4+T细胞中Th17细胞亚群的比例。同时选取30例健康人为对照。结果健康对照组Th17表达率为(2.41±0.62)%,脓毒症组(3.36±1.42)%,重症脓毒症组(5.33±2.67)%,脓毒性休克组(2.1±0.89)%,重症脓毒症组Th17表达率最高,脓毒性休克组与健康对照组差异不显著。结论脓毒症患者外周血Th17细胞表达增加,提示Th17细胞在脓毒症的免疫发病机制中可能起着重要作用。

Objective To study the level of Th 17 cells in peripheral blood of patients with sepsis and its significance. Methods Fiftyfive patients admitted to Emergency Department and Surgery ICU of our hospital in March-July 2009 were divided into sepsis group（n=18）, severe sepsis group（n=29）, and septic shock group（n=8）. Expression of Thl7 cells in CD4＋ T cells was detected by flow cytometry. Thirty healthy individuals served as controls. Results The expression rate of Thl7 cells was 2.41% ± 0.62%, 3.36% ± 1.42%, 5.33%± 2.67%, and 2.1% ± 0.89%, respectively, in control, sepsis, severe sepsis, and septic shock groups. The expression rate of Th17 cells was the highest in severe sepsis group with no significant difference between septic shock and control groups. Conclusion The expression of Th17 cells is increased in sepsis patients, suggesting that Th17 cells may play an important role in pathogenesis of sepsis.